The Fifth Kingdom  -  Chapter 11       

FUNGAL ECOLOGY

Hotlinks to: the dung succession - amphibious fungi in streams
aero-aquatic fungi in ponds - pine needle succession  
fire fungi - macrofungal ecology - fairy rings - the humungous fungus -
ATBI (All Taxa Biodiversity Inventory)

 

Ecology is the study of organisms as they relate to each other and their environment. It must be apparent that even in the taxonomic chapters I gave a lot of ecological information. Think of the effects that fungi have had on people: the potato famine, the downy mildew of the French grape vines, the blue mould of Canadian tobacco, the way chestnut blight removed an important species from the forests of eastern North America, and the more recent loss of the beautiful American elm trees to Dutch elm disease. Fungi may alter the ecology of our gardens, as their depredations persuade some people to give up growing roses (because of the prevalence of black spot disease, powdery mildew and rust) or phlox (because of its susceptibility to powdery mildew). The early leaf drop inflicted on horse chestnut trees by Guignardia blight (at least in Eastern North America) may persuade us to plant other shade trees. But in this chapter I want to explore some other areas of fungal ecology: some of the ways in which fungi influence the course of events in a variety of natural, as opposed to Man-made habitats. I will explore their roles in four natural habitats I and my undergraduate or graduate students have personally examined in some detail, and then give a few more general comments.


The Succession of Coprophilous Fungi

The first habitat is dung (which is also known by many other names). We may turn up our noses, but to some other organisms, dung is a considerable resource, which is constantly being produced in large quantities by billions of animals all over the world. You may think that because it has passed through an animal's digestive tract, every bit of nutritional value will have been extracted from it. This is a false impression. There may not be a lot of high quality protein left, but there is a great deal of microbial biomass, as well as many food components, for example, cellulose, that neither the animal nor its gut flora managed to digest. There are also excretory products which, though they are of no further value to the animal, are high in nitrogen: herbivore dung may contain 4% nitrogen -- more, in fact, than the plant material originally eaten by the animal. So, at frequent intervals throughout its life, every mammal evacuates from its gut a mass of first class fungal substrate, simply asking to be exploited.

Are there fungi which specialize in exploiting dung? And if there are, how do they gain access to this substrate when it becomes available? The answers may surprise you. About 175 genera of ascomycetes are largely or exclusively found on dung. The extremely advanced and successful agaric genus Coprinus has many species that occur exclusively on dung. There are also many specialized dung-inhabiting zygomycetes, among which Pilobolus and some of the elaborate anamorphs in the order Kickxellales are perhaps the most spectacular. So there is no doubt that a specialized mycota of dung-inhabiting (coprophilous) fungi exists.


But how do they compete successfully for this substrate? The answer here may be a little unexpected, but it is nevertheless perfectly logical. These fungi contrive to be first to exploit the dung by the simple expedient of being in it when it is deposited.

The only way to achieve that is to be eaten by the animal.

Coprophilous fungi manage this trick in several ingenious ways. These processes must take into account some immutable logic. 1) The fungi are growing in the dung and will therefore have to fruit on it. 2) Animals do not, in general, eat their own dung (though rabbits do, raising interesting questions about the coprophilous fungi associated with them). 3) Therefore, the spores must be somehow distanced from the dung in such a way as to increase their likelihood of being eaten by herbivorous mammals.

You have already read in earlier chapters about how several fungi of herbivore dung achieve this trick. How the zygomycete, Pilobolus, aims and shoots its sporangia up to 2 metres toward the light.

11 Pilobolus2.jpg (90556 bytes)

11-2 Pilobolus2.jpg (4776 bytes)

(photo above by W.R.West)

11-3 Pilobolus3.jpg (2696 bytes)

How the ascus tips of the apothecial ascomycete, Ascobolus, protrude from the hymenium and bend toward the light before shooting their spores.

11-17 Ascobolus4.jpg (4924 bytes)

How the necks of the perithecial ascomata of Podospora and Sordaria bend toward the light before their ascospores are expelled.
11-22 Podospora1.jpg (28908 bytes) 11-23 Sordaria.jpg (36153 bytes) 11-24 Podospora3.jpg (1830 bytes)
Each of these independently evolved phototropic mechanisms is obviously designed to direct the spores away from any other adjacent dung, and to increase the efficiency with which spores are deposited on nearby vegetation that has a good chance of being eaten by the animal.

Many other dung-inhabiting fungi are less specialized than those I have just mentioned, or have specializations so subtle that we have not yet detected them. Nevertheless, the fact remains that with patient and repeated examination, we can find a large number of fungi representing most of the major fungal groups on the dung of many herbivorous mammals.

Repeated observations will show that the various fungi tend to sporulate in a reasonably definite sequence. First the Zygomycetes will appear...Pilobolus [above], and other saprobic genera such as Mucor, Phycomyces, Thamnidium, Helicostylum and others .  Then the dichotomously branched sporangiophores of Piptocephalis [below] which is parasitic on some of the zygomycetes just mentioned...as are Chaetocladium and Syncephalis [below].

11-4 Piptocephalis1.jpg (4840 bytes) 11-5 Piptocephalis2.jpg (6850 bytes)
the tall sporangiophores of Syncephalis [below] with their swollen apices and linear merosporangia...
11-6 Syncephalis1.jpg (6743 bytes) 11-7 Syncephalis2.jpg (5339 bytes)
the graceful multiple recurved sporangia of Circinella minor (the three pictures below show a developmental sequence...note the columellas in the third picture.
11-8 Circinella1.jpg (4696 bytes)
11-9 Circinella2.jpg (5138 bytes)
11-10 Circinella3.jpg (5999 bytes)

11-11 Rhopalostylis.jpg (5054 bytes)

Rhopalomyces elegans [left], which parasitizes nematode eggs...

11-12 Cunninghamella1.jpg (3895 bytes)

11-13 Cunninghamella2.jpg (5675 bytes)

Cunninghamella with its apical vesicle and unispored sporangia...
Then the Ascomycetes..apothecial fungi like Ascobolus...zooming in from left to right...
11-15 Ascobolus2.jpg (6937 bytes) 11-16 Ascobolus3.jpg (7546 bytes) ascob3.jpg (4978 bytes)
Saccobolus...again zooming in from left to right
11-14 Ascobolus1.jpg (4635 bytes) 11-18 Saccobolus1.jpg (7990 bytes) 11-19 Saccobolus2.jpg (5031 bytes)
Thecotheus...here with 32-spored asci

11-20 Thecotheus1.jpg (8441 bytes)

11-21 Thecotheus2.jpg (5932 bytes)

and the perithecial Podospora and Sordaria,
accompanied by a variety of conidial anamorphs (Hyphomycetes) such as the blastic-sympodial Basifimbria...

11-25 Basifimbria1.jpg (6217 bytes)

11-26 Basifimbria2.jpg (4407 bytes)

...the nematode-trapping Arthrobotrys with its clustered didymosporous (2-celled) conidia and its various ways of snaring nematodes, including the 3-dimensional net shown here [below, right]...
11-27 Arthrobotrys1.jpg (6426 bytes) 11-28 Arthrobotrys2.jpg (3939 bytes) 11-29 Arthrobotrys3.jpg (5457 bytes)
the synnematal, slimy-spored (arthropod-dispersed) Graphium, here shown in a zoom sequence of four pictures...
11-30 Graphium1.jpg (4383 bytes) 11-31 Graphium2.jpg (6087 bytes) 11-32 Graphium3.jpg (4995 bytes) 11-33 Graphium4.jpg (5273 bytes)
the synnematal, dry-spored Cephalotrichum (below)...
11-34 Cephalotrichum1.jpg (6275 bytes) 11-35 Cephalotrichum2.jpg (5076 bytes)
...and Trichurus, a synnematal hyphomycete with twisted, hair-like setae arising all over the fertile head, which give it a 'big hair' look (below)...

11-36 Trichurus.jpg (4687 bytes)

and finally the Basidiomycetes, mainly small (but profuse) species of Coprinus...

11-37 Coprinus1.jpg (32485 bytes)

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11-38 Coprinus2.jpg (4550 bytes)

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11-41 Coprinus5.jpg (5960 bytes)

It has been suggested that this is a true ecological succession, albeit a miniature and condensed one. Initially it was postulated that the sequence was a nutritional one. Zygomycetes can generally assimilate only fairly accessible carbon sources, such as sugars. Their fast growth was assumed to give them an advantage in finding these, and their early disappearance was thought to be due to the exhaustion of this substrate. The ascomycetes and conidial anamorphs that appeared next were assumed to be able to assimilate more complex carbon sources such as hemicellulose and cellulose; while the basidiomycetes, appearing last and persisting longest, were able to exploit both cellulose and lignin.

But when this hypothesis was scrutinized more carefully and tested by experiment and further observation, it did not hold up. The growth rates of the various fungi were found to be relatively similar, and the various carbon sources were not exhausted as quickly as had been assumed. So a second hypothesis was advanced. This one was based on the time it took for each kind of fungus to accumulate enough food reserves to permit it to fruit. It was argued that the simple sporangiophores of the zygomycetes could be developed after only a short period, while the more elaborate fruit bodies of the ascomycetes would require a longer build-up, and the even larger basidiomata of the coprini would need the longest preparation of all. This is a more reasonable hypothesis, because if we grow some of the dung fungi on laboratory media, we find that it takes Mucor hiemalis 2-3 days to sporulate, while Sordaria fimicola needs 9-10 days, and Coprinus heptemerus 7-13 days.

Some of the Kickxellales, zygomycetes often found on the dung of sedentary mammals (those with a defined home base, a small territory, and habitually used paths) produce extremely complex and convoluted anamorphs.

Spirodactylon, possibly the most complex of all, produces tall, branched sporangiophores that bear tiny coils within which develop innumerable one-spored sporangia. The whole structure must be designed to catch on the hairs of the rat or mouse as it passes by. This is made possible by the habits of the animal which, although it doesn't eat its own dung, at least deposits it somewhere along one of the trails it follows every day in its journeys to and from its den or burrow. The final step, the ingestion of the spores, is presumably taken when the animal grooms itself, as mammals (other than human children) habitually do. Some coprophilous hyphomycetes (e.g. Graphium) produce slimy droplets of conidia at the top of tall conidiophores or synnematal conidiomata. These spores are presumably dispersed by arthropods which may themselves specialize in seeking out dung, and may thus act as specific, and very efficient, vectors for the slimy-spored fungi.

Many other dung-inhabiting fungi are less specialized than those I have just mentioned, or have specializations so subtle that we have not yet detected them. Nevertheless, the fact remains that with patient and repeated examination, we can find a large number of fungi representing most of the major fungal groups on the dung of many herbivorous mammals. Repeated observations will show that the various fungi tend to sporulate in a sequence. First, zygomycetes will appear; then ascomycetes and conidial fungi, and finally basidiomycetes.

So we can assume that an assortment of spores of coprophilous fungi will be present in dung when it is deposited, and that these will all have been triggered to germinate by some aspect of passage through the mammalian gut. While Pilobolus is producing its miniature artillery extravaganza, the other fungi are growing and assimilating steadily within the dung, preparing for their own appearance at the surface. The new hypothesis had neglected only one important factor: antagonism. After a few weeks, almost the only fungi still sporulating on the dung will be species of Coprinus. These can go on producing a sequence of ephemeral basidiomata for months. We now know that the various components of the substrate are far from exhausted after the initial flushes of growth and sporulation. What has really happened is that Coprinus has seized control by suppressing most of the other fungi. Hyphae of Coprinus are actually extremely antagonistic to those of many other coprophilous fungi. If a Coprinus hypha touches one belonging to Ascobolus, the Ascobolus hypha collapses within minutes. We don't understand exactly how this trick is done, but it is extremely effective, and turns out to be a fairly common stratagem among the fungi, whose main competitors for many substrates are other fungi

Another interesting and important gambit used by Coprinus involves repeated anastomoses. Spores are more or less evenly dispersed throughout the dung when it is deposited, and they all germinate more or less simultaneously, producing small mycelia within the dung. When compatible mycelia meet, they will anastomose, and soon the entire dung deposit is permeated by what is now essentially a single mycelium, which can then pool its resources and produce more and larger basidiomata. Cooperation pays off for Coprinus.

11 Coprinus.gif (9697 bytes)


There are also some interesting subplots that run concurrently with the main story. Several of the zygomycetes that usually appear (e.g. Piptocephalis) are actually parasitic on other zygomycetes. One common zygomycete, Rhopalomyces elegans, parasitizes nematode eggs. Nematode-trapping fungi such as Arthrobotrys often sporulate, and develop their characteristic rings and nets (see Chapter 15). Keratinolytic hyphomycetes such as Microsporum (below) may appear on hair that the animal has accidentally eaten during grooming.

onygen7.jpg (5305 bytes)

Occasionally, an undescribed species of fungus may be seen. For many years the third year mycology class at Waterloo followed the dung succession as a laboratory exercise. These undergraduates saw the zygomycete Stylopage anomala on horse dung several years before it was formally described in 1983. They also found an undescribed species of Podospora (Ascomycetes), which is perhaps the 102nd species of this genus. They also found the rare zygomycete, Helicocephalum, which I had never seen before.

Horse dung is easy to obtain in most areas, comes in discrete units, and can be handled and observed without creating much personal distress. As many as 40 species of fungi representing most major groups of eumycotan fungi are commonly recorded from a single collection of horse dung. Most of them can be identified fairly easily with the help of the specialized taxonomic literature that is now readily available, though I admit that some of the zygomycetes are not easily recognized as such by beginners. Most of them could be identified to genus with the help of the illustrations on this CD-ROM.  Many of the fungi can be isolated in pure culture without too much difficulty, and with a little imagination, interesting experiments can be devised to investigate various aspects of their behaviour. Perhaps now you can understand why I and many other teaching mycologists ask our classes to put their culturally determined attitudes on hold, adopt an objective scientific approach, and study the succession of fungi on horse dung, then think about the biological mechanisms and manoeuvring that lie behind the visible manifestations. It's a truly thought-provoking mycological experience.

Before we leave this topic I should warn you that you should not collect and examine the dung of carnivores, because it might support fungi that could also grow on you.  However, in the interests of science, your humble scribe checked out the dog do-do shown below, which had taken on a transmogrified appearance in cool, humid weather. I found that the principal fungus fruiting here is a species of Mucor (Zygomycetes).  I was probably quite safe, because the animal was eating kibble, not cats.  But my advice is still the same: stick to herbivores...their droppings don't smell nearly so bad... 

If you want to know more about the coprophilous fungi, I recommend that you read a recent paper:  Richardson, M.J. (2001) Diversity and occurrence of coprophilous fungi.  Mycol. Res. 105: 387-402.            

I cannot close this section without mentioning a magnificent new book from an Italian physician, Dr. Francesco Doveri (see references).  It has 1104 pages, 158 colour photographs and 300 other illustrations.  It is undoubtedly the most extensive coverage yet afforded to coprophilous fungi, and took the author 15 years to bring this project to fruition.   Definitely worth a look, if you can find a copy.

Now on to a very different habitat...


Amphibious Fungi in Streams

The second area of fungal ecology I want to examine is a stream flowing through a woodland, somewhere in the temperate zone. We already know that the tiny chytrids and oomycetes live here, but we might not expect to find many of the typically terrestrial dikaryan fungi. However, if you collect some stream foam and examine it under the microscope, you will see that the bubbles have trapped a rather unusual kind of spore (this is simply a physical phenomenon -- a surface tension effect -- and there is no other relationship between the bubbles and the spores).

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11-42 bubble spores.jpg (4792 bytes)
I collected foam in winter from this stream near where I live...  

If you pass a litre of this stream water through a filter, then stain the filter in cotton blue and examine it through the microscope, you will see many large and strikingly shaped fungal spores. Many, perhaps most, will be tetraradiate.
These two sets of drawings are from a booklet published by Ingold (who discovered these strange fungi) in 1975.
You can see tha there is a wide range of different morphologies, almost all of which share one feature - they have arms or appendages sticking out in various directions. 

We will see how these evolved...

11-43 Tetrachaetum spores.jpg (5200 bytes) dark field picture of Lemonniera conidia
11-45 Lemonniera spore.jpg (3728 bytes) phase contrast photomicrograph of a conidium of
Lemonniera aquatica
Clavariopsis aquatica
(phase contrast)
Tetracladium marchalianum (interference contrast)
Articulospora tetracladia
(phase contrast)
Culicidospora gravida (phase contrast)
Others will be unbranched, long, thin and arc-shaped, sinuate or sigmoid (s-shaped).
Anguillospora, a common sigmoid form (phase contrast)
They are all produced by conidial anamorphs that are specially adapted for living in streams.
There are even yeasts with a tetraradiate arrangement of their cells, presumably for the same reason this shape has been adopted by the other spores.  This photomicrograph is of Candida aquatica.
Where do these spores come from, and how do the fungi that produce them make a living? The first clue came when limnologists (biologists specializing in freshwater systems) began to examine the energy budgets of streams. Because some streams flow through forests, they are heavily shaded during the growing season. This means that few green plants (primary producers) can grow in them. It was found that more than half, and sometimes nearly all, of the energy supporting organisms that live in streams comes from autumn-shed leaves. This source of energy is described as 'allochthonous' (which means 'coming from somewhere else' just in case you wanted to know).

When they first fall into the water, these leaves are extremely unpalatable to stream invertebrates, but as they are colonized and 'conditioned' by microorganisms, they apparently become tastier.  Experiments in which batches of leaves were treated with either antifungal or antibacterial antibiotics showed that the fungi were chiefly instrumental in making leaves palatable to animals such as Gammarus pseudolimnaeus, a numerous amphipod crustacean living in the stream (another amphipod lives on the beach below my house in millions, eating decaying tidal jetsam, mostly seaweeds and, no doubt, the fungi growing on and in them).
11-49 Gammarus.gif (9274 bytes) Gammarus, a detritivorous and mycophagous amphipod crustacean
11 gammarus feeding expt1.jpg (2575 bytes) In a feeding experiment, Gammarus (the dark, comma-like objects) choose to eat  fungal mycelium (the greyish stuff at lower right) rather than unconditioned leaf discs (dark circles).
Later experiments with leaves conditioned by individual stream fungi showed that not only were some of the fungi that produce tetraradiate or sigmoid conidia most active in conditioning leaves, but their mycelia and sporulating structures were also highly nutritious food for detritivorous stream animals such as Gammarus (Amphipoda, Crustacea). An important ecological role had been established for these fungi.
But many questions remained. Were those fungi with tetraradiate spores related to one another? Did they have teleomorphs? (which would help to answer the first question). Since streams always flow the same way, and have a natural tendency to carry small things like spores downstream, where did the inoculum for the upper reaches come from? What were the advantages of the tetraradiate and sigmoid spore shapes? The information we needed was gradually accumulated over several years of experiments, until eventually we were in a position to give some answers.

Many of the tetraradiate (4-armed) spores, though similar in configuration at maturity, developed in rather different ways. I will describe just two of these. In some, three arms grew upward and outward from the top of the first-formed arm. In others, one arm grew upward, the other three or four outward and downward at the same time from a central cell. Some of these conidia were thallic, some blastic. A few had clamp connections, like Taeniospora gracilis, shown here, and were clearly basidiomycetous...

but most didn't. This impression of diversity was confirmed when some of the teleomorphs were discovered. Some were unitunicate ascomycetes, both operculate and inoperculate, producing apothecial and perithecial ascomata. Some were bitunicate ascomycetes. Some were basidiomycetes.

For some recent conclusions based on molecular research, click here.

It became clear that the morphologically similar anamorphs were actually a mixed bunch: fungi of very different origins that had undergone convergent evolution, molded by selection pressure into similar shapes. The teleomorphs also provided one answer to the question of how these fungi got upstream: ascomata and basidiomata, unlike the anamorphs, were not submerged in streams, and they liberated airborne ascospores or basidiospores. The group has been christened the amphibious fungi, because of its immersed anamorphs and emergent teleomorphs.

But why did so many of these taxonomically diverse amphibious fungi evolve conidia with similar shapes? It was found that as they were carried along by the water, tetraradiate spores sometimes entered the layer of still water just above the surface of submerged leaves, and then made three-point landings on these leaves. We know that a tripod is the most stable configuration, able to stand firm on irregular surfaces. The spores formed microscopic tripods that gave them a foothold on the dead leaves for long enough to germinate from the ends of the three arms, and attach themselves to the substrate before being swept away.

Much of the early work on stream fungi was done by Terence Ingold, who published many papers on the strange fungi to be found on leaves in water, starting as long ago as 1943.

11-46 3pointlanding.jpg (4077 bytes)

The reason for the sigmoid shape has not yet been fully established, but Webster and Davey (1984) published a paper: 'Sigmoid conidial shape in aquatic fungi'  in Transactions of the British Mycological Society 83: 43-52.  They observed that most such conidia tended to roll along in flowing water with their long axes at right angles to the current, though some vaulted end over end when they touched a surface.  Both kinds of movement bring the ends of the spore into contact with the substrate.  If the flow slows down (and it is almost zero at the boundary layer next to the substrate), this gives the end of the conidium a chance to stick to the surface.  After attachment, the conidia swing around and lie with their long axis parallel to the current, and rarely become detached again. They then produce sticky appressoria, and germinate quite quickly, apparently stimulated by the contact.   

Sigmoid conidia may represent an evolutionary compromise.  Although not as efficient at attachment as tetraradiate spores, they represent a more efficient allocation of resources. As usual in the living world, there is more than one answer to a particular problem

After colonizing the leaves, the amphibious fungi sporulate again, and it was found that they would do this only in highly oxygenated conditions, and with the physical stimulus provided by flowing water.  It is clear that amphibious fungi incorporate many special adaptations, both morphological and physiological, to their environment.

11-47 Lemonniera sporulating.jpg (5051 bytes)

If the spore numbers are charted over the entire year, it will be seen that their numbers peak in Fall and Spring. In the first place, the massive new input of autumn-shed leaves provides the necessary substrate. In the second case, spring run-off will also carry plant debris into the stream. The entire process is diagrammatically summarized below, showing that the fungi are vital intermediaries of energy flow in streams, providing a link between dead leaves and trout.

11 stream diagram.gif (30018 bytes)

Nikolcheva et al. (2005) have used molecular techniques to explore diversity of fungi in the early stages of colonization of leaves in streams (see references), and showed that after initially high diversity, numbers of taxa fell as terrestrial fungi were outcompeted by aquatic species, and aquatic species established their own pecking order.

Aero-Aquatic Fungi in Ponds


One good aquatic habitat deserves another, so after sorting out the role of fungi in streams, we switched our attention to woodland ponds (our third habitat).  The pond in question lay in the heart of the woods behind my house in Waterloo.
Again, primary production within the pond was limited by the forest canopy. Again, there was a specialized group of fungi living in the pond, though no-one knew if these fungi played an important role in the ecology of the pond. In this case the fungal propagules commonly found were hollow, and floated. Again, this end was achieved in several different ways, of which I will describe only two. The pond has almost dried out in summer (1) A conidiophore emerges from a dead leaf just below the surface of the the water, and branches like a tree. Eventually, the ends of the fine branches all swell up and fuse with their neighbours to form an air-filled, watertight structure. This is the propagule of Beverwykella.
11-60 Beverwykella1.jpg (6567 bytes) 11-61 Beverwykella2.jpg (5880 bytes)
(2) Another conidiophore grows from a dead leaf, emerges through the water surface, and its tip begins to grow in circles. Coiling repeatedly on itself in wider and wider, then narrower and narrower gyres, it eventually builds a barrel-shaped, air-filled, watertight structure. This is the propagule of Helicoon.
11-56 Helicoon1.jpg (5117 bytes) 11-57 Helicoon2.jpg (6300 bytes)
11-58 Helicoon3.jpg (8708 bytes) 11-59 Helicoon4.jpg (5216 bytes)
11 Limnoperdon plate.jpg (50561 bytes) Here  is another apparently rare pond fungus, the tiny floating gasteromycete, Limnoperdon.  It has been recorded only from our pond in Ontario and somewhere near Seattle, Washington, though it surely occurs at many places between those widely separated localities -- people just haven't looked carefully.

The fruit bodies (magnified X 10 in the top left picture, and X 50 in the top right picture) are hollow, and are lined with non-shooting basidia (bottom right X 1500).  Note the symmetrical mounting of the spores and the lack of a pointed sterigma (see discussion in Chapter 5b).
A young basidium (bottom left) shows the typical clamp connection at its base.


Because these fungi live and grow under water, but produce their spores only above the surface, they are called the aero-aquatic fungi. It's obvious that the structures of the two kinds of conidia  described above, though functionally equivalent, are not closely related. Again, convergent evolution has been at work, the selection pressure applied by some ecological imperative.

We finally discovered what this was.  It was the need to be first on the scene when new substrate appears. When a dead leaf falls into a pond, it does not sink immediately.

It may actually fall on top of some of the floating propagules just illustrated, or the propagules may be drawn to the floating leaves by surface tension. In either case, these fungi will be the first pond-adapted species to enter this new substrate. The leaves soon sink to the bottom of the pond, carrying their new colonizers - hyphomycete or gasteromycete - with them

These fungi also have the ability to grow at low oxygen levels, and to survive the virtually anaerobic conditions that prevail at the bottom of a pond for extended periods during the winter.

Sporulation will happen again when the pond begins to dry out during the following summer, and the water level subsides until the colonized leaves are once more just below the surface. We found that these aero-aquatic hyphomycetes play an ecological role parallel to that of the amphibious fungi in streams: conditioning the dead leaves, and making them palatable to the detritus-eating invertebrates such as snails, and vertebrates such as frogs, whose larval stages live in the pond.
Frog spawn [left]
produces tadpoles which skeletonize leaves after the fungi have 'conditioned' them...
...and eventually metamorphose into tree frogs [left] which represent the apex of the pyramid of life in the pond.
There are quite a few marine fungi, including many ascomycetes, some hyphomycetes and a small number of basidiomycetes, but all the evidence, both morphological and molecular, points to terrestrial origins for these fungi.
 
Other Habitats

The biosphere has myriads of other habitats, each unique in various ways, and each making special demands of the organisms that live in it. The roots of plants create special conditions around themselves, and have established especially intimate relations with hundreds of endotrophic and thousands of ectotrophic mycorrhizal fungi (which have Chapter 17 to themselves). Other rather less specialized saprobic and parasitic fungi also abound on and near roots. The surface of living leaves is inhabited by a specialized mycota, while dead and decaying leaves are substrates for a succession of other species. The soil, into which most leaf remains are incorporated, is itself a mass of microhabitats, and is the richest reservoir of fungal diversity. And of course the leaves of different plants, and the various soil types, will have different subsets of the total mycota.    Juliet Frankland, in her Presidential address to the British Mycological Society (reference below), gives a nice overview of the problems and progress in our study of fungal succession, exemplifying them with an autecological study of the agaric, Mycena galopus.

Not all fungi can be parceled out neatly into successive steps of a succession. Often, fungi compete for access to a substrate. Sometimes a natural phenomenon will give us an unexpected insight into this struggle. Here is a picture of a block of wood which has been colonized by many different mycelia. The boundaries between the 'territories' of different mycelia can be clearly seen as black lines or zones, and the wood is described as 'spalted.'  The black material is melanin-like, oxidized and polymerized phenolics deposited by wood-rotting fungi, and although the biological function of the zones isn't entirely clear, melanins are the precursors of the humic acids, which are long-lived and important determinants of soil fertility.

This kind of partition can even occur in much smaller substrates, such as individual leaves, as in this one of Oregon grape (Mahonia) from John Dean Provincial Park near where I live.  The colonies and black fruit bodies shown are of Coccomyces (Rhytismataceae, Ascomycetes).  The lower pictures are close-ups, which show the unusual polygonal outline and stellate opening of the ascomata. Note that while the colonies in the second picture have produced only one ascoma each, that in the third picture has a larger territory, and has been able to develop multiple ascomata, now open to expose the hymenium.

11 leaf zones.jpg (49883 bytes)

Another fungus exploiting leaves of Mahonia is Cumminsiella (Pucciniaceae, Teliomycetes). 

This fungus attacks living leaves, and thus preempts the saprobic Coccomyces.
This time a single infection occupies a whole leaf. The uredinial sori open on the lower surface of the leaf.

There's more information on this fungus, including a photomicrograph of urediniospores and a teliospore, in Chapter 5d.
This senescent salal (Gaultheria shallom) leaf has also become divided up, but in this case two or three different fungi are involved.

Even if a leaf isn't subdivided into territories, after it dies you are likely to see fungal colonies develop, as is happening in the Hosta leaf (above, left) from our garden. The fungi in this case are mainly Cladosporium and Epicoccum, two common saprobic hyphomycetes.  The picture on the right shows a part of another Hosta leaf, clearly demonstrating that the areas covered by the fungal colonies, marked X, are the first to be eaten by animals. We may compare the fungi to peanut butter, and the leaf to the bread which it renders palatable.

Which brings me to the subject of my own PhD thesis -- the succession of fungi involved in the long, slow decomposition of another kind of leaves -- Scots pine needles (our fourth habitat).

The pine needle microsere

I was presented with a problem which, briefly stated, was as follows.  "When we isolate fungi from the soil, the majority of cultures will be of light-coloured fungi, while a majority of the hyphae seen in the soil are darkly pigmented.  Figure out what's going on."

I chose to work in a pure stand of Scots pine (Pinus sylvestris)

I looked at the various soil horizons in the forest, and tried innumerable times to grow the dark hyphae, picking them out with a micromanipulator and giving them a variety of delicious media.  But they refised to grow, so I eventually decided that most of them must be dead, and that they had perhaps grown at some other time and in some other place.  I looked in the organic horizon above the mineral soil, and found there a thriving community of litter decomposing fungi, which I proceeded to investigate (I did not realize it at the timer, but this is fairly typical of  PhD projects, which are often changed in mid-course by some unforeseen event(s).
11-66 horizons.jpg (132504 bytes) I embedded a small chunk of the Scots pine forest floor in cold-setting resin, then cut it into vertical slices, one of which I drew for this illustration, which shows the spatial distribution of needles, and their gradual transition from L >  F1 > F2 > H layers. I then decided to examine as many needles from each layer and sub-layer as I could process each month (the number turned out to be 300 needles).
11-67 needle categories.jpg (6322 bytes)   The left-hand vial contains living needles from the tree, which represented stage one in fungal colonization.  Vial 2 = L layer (pale brown), vial 3 = upper F1(much darker, but still tough), vial 4 = lower F1 (blackish and softer), vial 5 = F2 (greyish and fragmenting). By the time litter material entered the H layer, it was no longer recognizable as individual needles. 
Needles were treated in various ways. (1) Some were washed repeatedly to remove loose surface spores and plated out in segments to isolate fungi on and in the needles. (2) Some were surface sterilized before plating out, to select for internal colonizers. (3)   Some were wax embedded and sectioned. (4) Some were observed directly over a period of incubation in damp chambers.  
11-68 living needle ts.jpg (7417 bytes) This is a reference point -- a transverse section of a  healthy, living needle.  Changes can be measured against this.
phacid6.jpg (2899 bytes) And here is one of the first dramatic changes, the development of numerous ascomata of Lophodermium pinastri, which apparently often colonizes the interior of living needles without producing overt symptoms. The death and fall of the needle stimulates the fungus to fruit.
11-69 Lophodermium needle ts.jpg (7322 bytes) The large number of lenticular black ascomata of Lophodermium that can occur in a single needle indicates a dominant colonizer. Here two ascomata are seen in section.
11-70 Fusicoccum needle ts.jpg (6855 bytes) Other fungi fruit in other needles -- note the several paths along which individual needles may travel.  In this picture, the fruit bodies are pycnidial conidiomata of a coelomycetous anamorph, Fusicoccum (holomorph probably Botryosphaeria).   The interior of the needle can be seen to be breaking down under the attacks of the fungus.
11-71 surface reticulum.jpg (8704 bytes) Meanwhile, on the surface of the needle, networks of dark hyphae (remember them?) develop. But what fungi do they represent?  Mycelium without sporulating structures is not very helpful unless one has access to molecular techniques.
11-72 Slimacomyces reticulum.jpg (5804 bytes) Fortunately, several of these fungi fruited either in nature or in the damp chambers.  The first of these is Slimacomyces monospora (which I mistakenly described as a species of Helicoma in 1958!)
11-72a Slimacomyces.gif (5438 bytes) Here is a drawing of Slimacomyces monospora, with its single helicosporous chlamydospores
11-73 Sympodiella cphs.jpg (2715 bytes) A second major surface colonizer is Sympodiella acicola which I described as the type species of a new genus (It still stands). Again, note that the conidiophores are in an almost pure stand.
11-73a Sympodiella.gif (4318 bytes) Here is a drawing of Sympodiella acicola, showing that its unique characteristics are that while its conidiophore extends sympodially, the conidia are thallic-arthric (for those of you who are fans of conidium development -- otherwise look back to Chapter 4).
11-74 Thysanophora sclerotia.jpg (5938 bytes) Another fungus that is obviously at least partly internal develops sclerotia and conidiophores.  This was Thysanophora penicillioides...
Thysanophora.jpg (9184 bytes) And here is part of a Thysanophora conidiophore with a penicillus (the brush-like conidiogenous apparatus - the numerous cells at the top are phialides, which can each produce many conidia in a dry chain).
11-75 Verticicladium.jpg (3772 bytes) Another pure stand of external conidiophores of an internal fungus, Verticicladium trifidum (an anamorph connected to an apothecial fungus, Desmazierella acicola, which I never saw). The conidiogenous cells of this fungus extend sympodially during conidiogenesis.
11-76 needle zones.jpg (4251 bytes) Finally, we have arrived at the partitioning of needles among fungi.   It is particularly obvious here, where a darkly pigmented fungus squares off against an apparently unpigmented fungus.
11-77 internal zones.jpg (5712 bytes) Section of a partitioned needle, showing the melanin barrier between species.  The fungus at upper left is Verticicladium; its neighbour is not fruiting so cannot be identified.  Compare this partition with the black lines shown earlier in wood and leaf.
11-79 cavity faeces.jpg (7414 bytes) Now a new participant bursts onto the scene (well, it's aleady left, but it has left its trademark - frass).  Now the needles have been softened up by the fungi, arthropods can eat the needle material.  The frass identifies the intruder as an oribatid mite.
11-80 beetle mite.jpg (8114 bytes) And here it is, a miniature armoured tank that eats fungi and needle.
11-81 surface deposits.jpg (7665 bytes) Now in the lower F1, the interior of the needle has collapsed or been eaten, and the upper surface is coated with a deposit of frass, which contains many fragments of fungal hyphae and spores.
11-82 succession diagram.gif (19687 bytes) This diagram plots the overall picture, following the needles through 9 years of mainly fungal decay.  The width of each bar represents the relative importance of the fungus at each stage. Darker bands show fruiting periods. At far left the fungi are those that grow on or in living needles. As we move to the right, the fungi involved in later stages of decay are traced.  
11-83 soil organism biomass.jpg (20943 bytes) Read this table carefully -- it will amaze you, and it shows just how important fungi are in the forest ecosystem.  Not merely important, but producing greater biomass than any group other than the plants.

A recent paper by Paulus et al. (2003 - see references) gives further insight into what is going on in decaying plant litter.   Using a particle filtration technique, these authors isolated no fewer than 1365 isolates, representing 112-141 morphotaxa, from 8 leaves of Neolitsea delabata in the tropical forests of Queensland. 
 

Fire fungi

Forest fires, slash burns, camp fires and even volcanic eruptions will all trigger the fruiting of a rather specialized group of macrofungi. We are all becoming familiar with the massive fruitings of morels, particularly the black morels, Morchella elata and allies, which appear in the spring on areas burned the previous year. Foresters may be familiar with the ascomycete, Rhizina undulata, which resembles, quite literally, a pile of dung, because this species attacks conifer seedlings on burn sites. Less familiar are the many other species in the genera Pholiota, Myxomphalia, Omphalina, Tephrocybe, Psathyrella, Coprinus, and the cup fungi Pyronema, Lamprospora, Octospora, and Peziza that appear in similar situations. While some of the cup fungi and Pholiotas are brightly coloured and conspicuous, others are black, dark grey or brown, and therefore are hard to see among charred wood and soil. If you want to find these fungi, squat down and slowly scan small areas, particularly those showing some regeneration of mosses. Many of the ‘fire fungi’ are in fact bryophilous species associated with the mosses and liverworts that also characterize old burns. Fire fungi may be induced almost anywhere, by controlled burning (and a little patience), or in the laboratory by various heat shock treatments of soil samples.


Other special substrates have evoked their own suites of specialized fungi: keratin is attacked by some of the Onygenales (Ascomycetes) and their anamorphs; wood by many Aphyllophorales and some Agaricales.  Extreme physical conditions (see fire, above) have selected specialist fungi which, by evolving the ability to cope with high or low temperatures, or low water activity, have essentially escaped from competition, and gained access to untapped food supplies. Some fungi are the most osmotolerant organisms known (see Chapter 20). The cycling of anamorph and teleomorph, which I mention many times in connection with plant disease fungi in Chapters 4 and 12, is often largely a matter of their response to specific ecological conditions, which turn on and off large segments of the genome. The fungal ecology of sewage, compost, mushroom beds, agricultural and forest soils, naturally decomposing plant remains, some cheeses, bread, wine and beer, crops in the field and after harvest, the air, the space between your toes, and the tissues of immune-deficient or immune-suppressed people: all can be the subjects of worthwhile, and even important, studies of fungal ecology.

Many of the food webs illustrated in ecology textbooks miss out more than half of the organisms involved in the transfer of energy and nutrients. They often stress macroscopic organisms, while omitting microscopic organisms such as the saprobic and mycorrhizal fungi. This neglect is unfortunate, especially since we now appreciate that microorganisms, being at the base of food webs, provide nutrients and mutualistic symbionts for almost all plants and animals. The basic links in terrestrial food webs lie in the soil which is, of course, where a huge number of fungi still live. Every attempt to understand trophic systems must start and finish with soil organisms. And surely the fungi are among the most important of those.

Macrofungal Ecology - Help wanted!

Most of the situations I have described in this chapter are small or localized.  If we consider the macroscopic fungi, and their roles in such extensive ecosystems as forests, we find that the state of fungal ecology is relatively primitive, meaning that we simply don't know very much about how those fungi act and interact under natural conditions.  If you doubt this, you could explore the mycological literature for information on where to find morels (in my opinion, the best of all edible fungi).  You will be led a merry dance, from old apple orchards and dead elms to recently burned forests.  Until relatively recently, no-one even seemed to know whether morels were mycorrhizal or not (my understanding is that they are opportunistic saprobes, exploiting new substrates then fading away, only to appear somewhere else when new food sources present themselves). 

As for the ubiquitous agarics, which are undoubtedly the most widely collected and studied of all fungi, I have to report that things aren't much better.  Only Europe holds out a candle in the darkness.  Since europeans have been collecting and recording macrofungi for centuries, they have the kind of database that allows the present generation of mycologists to draw comparisons with the past.  This is why several European countries have 'Red Lists' compilations of macrofungi which seem to have undergone serious declines in recent years -- or even to have disappeared altogether.  It is impossible to produce such red lists for anywhere in North America because records do not go back far enough and are, in any case, still fragmentary.  Although we may suspect that certain species are declining or disappearing, we have no well-documented historical reason for saying so.  You can find out more about red lists by going to a good search engine such as 'google' ( www.google.com ) or 'Yahoo' or 'alltheweb' and entering 'red list endangered fungi'.

We understand that about half of the known agarics are mycorrhizal -- they have an intimate, mutually beneficial relationship with many of our forest trees, and ecological research has recently begun to focus on the effects on such fungi of various forest practices, and especially the clear-cutting of old-growth forests which still (regrettably) goes on in many jurisdictions, and most blatantly in British Columbia where I live.  One of my own graduate students has recently established that many of the fungi associated with old growth forests do not re-colonize clear-cut  habitats for 40-50 years.  And his suspicion is that the recolonization happens by means of airborne basidiospores which originate in nearby old growth.  What if there is no longer any nearby old growth to provide this inoculum?  But International logging companies carry on in blissful ignorance of any such concerns.  

Just when we think we have established a few principles based on the occurrence of fruiting bodies of the mycorrhizal fungi, it is demonstrated by molecular techniques that in many cases the fungi producing the fungal sheaths around the roots of the trees are not those whose basidiomata are appearing above ground.  Are we back to square one?  No one seems sure at present.  But I mention this to demonstrate how little we actually know about macrofungal ecology.   Some recent studies (2005) claim that the apparent disconnect is at least partly because many of the mycorrhizal fungi develop small, inconspicuous, cryptic or seasonally limited  fruit bodies, rather than the large and conspicuous ones detected by most collectors.

A fascinating study by Tofts and Orton (1998) points out that although they have been collecting agarics regularly in a particular woodland in Scotland for 21 years, and have recorded 502 species in that time, each year they still find species they have never seen before.  Over twenty years of collecting, and they still cannot say that they have a proper handle on agaric biodiversity in that woodland.  They suggest that at least 25 to 30 years of collecting, and possibly more, will be necessary before that goal can be attained. 
An even more recent paper by Straatsma, Ayer and Egli (2001) emphasizes many of the same points.  They collected basidiomata weekly for 21 years (1975-1999, except for 1980-1983), and recorded over 400 species in a 1500 m2 plot.  Yet only 8 species were found every year.  The number of species/year ranged from 18 to 194, and even in the last year of the study, 19 species appeared which had not previously been found.  Clearly, the authors had not seen the full diversity of macrofungi.

A group of west-coast mycologists (including Paul Kroeger, Christine Roberts, Oluna and Adolf Ceska, and me), supported by the Mellon Foundation, has been doing a macrofungal inventory of Clayoquot Sound on the west coast of Vancouver Island.  Over 5 years, visiting once in spring and twice in fall, we have collected 660 species.  Two of the most interesting features of our study have been: (1) that only 38 species were found every year and 407 were found only once, and (2) that a large number of fungi new to the study  cropped up each year.  After year one, we have found about 100 additional species each year. 

In Fall 2004 the Cascade Mycological Society held its 16th successive mushroom fair at the Mount Pisgah Arboretum just outside Eugene, Oregon. As a guest speaker for the Society I was fortunate enough to be invited to participate in the collecting trips leading up to the fair. The fair is an exciting introduction to the larger fungi because over 300 species are usually on display - speaking to a huge effort on the part of the members. I was also fortunate enough to get my hands on the statistics for all sixteen years. Over those years just over 700 species have been recorded from the area. When we arranged the data according to the number of years in which each species had been collected, an interesting picture emerged.

To begin with the extremes. I was rather surprised to discover that only 37 species (just over 5% of the total number) had been found in all sixteen years. It was equally thought-provoking to learn that no fewer than 190 species (almost a third of the total) had been recorded only once in those 16 years.  Here is the list of species versus years.

Species       No. of years recorded

  37                       16
  44                       15
  31                       14
  24                       13
  20                       12
  13                       11
  18                       10
  18                         9
 
22                         8
  22                         7
  22                         6
  41                         5
  29                         4
  48                         3
  92                         2
190                         1

There are possible flaws in the data set. For example, species may have been misidentified. But the general trend is obvious. A relatively small number of taxa will show up every year, or almost every year, while a much larger number of taxa will be found much less often, and a very large number will be encountered only once every decade or so.

How many more taxa will show up in the years to come? What is the full number of species that the Cascade group can expect to find if they keep at it long enough? If we may be allowed to take a quick look in the crystal ball, might we not find that after 50 years they will have found 1,000 species?

This data set (for which I am indebted to the hardworking collectors and record-keepers of the Cascade Mycological Society) points up the necessity for very long-term studies wherever the diversity of fungi is to be fully explored, and calls for the accumulation of much concurrent data on weather conditions and other ecological factors if we are to understand why some fungi are so notably shy.

These reports are not intended to put you off, to deter you from getting involved in fungal ecology.  Rather the reverse.  It is clear that the need for research in this area is critical.  We need good ecological studies just as much as we need molecular research on fungi.  Some groundwork has been done in Britain, where the macrofungal assemblages characteristic of many habitats have been broadly outlined.  But this is still far from an understanding of the full role played by those fungi in the habitats being considered.  The need for seminal research has never been greater.  The next section discusses some of that.

Fairy Rings

Fairy rings are one of the few fungal phenomena that most people have seen. To the superstitious mind the arrangement of the fruit bodies in a circle might seem very strange. On top of that, the grass often doesn’t grow just inside the fruiting zone, so it looks really weird, and it isn’t too surprising that in the past, people ascribed such rings to supernatural events (fairies or witches dancing).

The real explanation is simple enough, once you know that mushrooms start life as microscopic spores that germinate, develop branching hyphae, and soon grow outward from their point of origin as a mycelium that tends to spread equally in all directions (see Chapter 3a), and thus forms a colony that describes an ever-widening circle in the soil. When the mycelium has accumulated enough food, and conditions are right, mushrooms develop at the periphery of the circle, finally bringing the previously invisible colony to our attention.

Fairy rings become larger every year, as the mycelium grows outward, always looking for food. It can’t turn back because it has used up the food resources behind it.  Growth of the  grass is inhibited because the dense fungal mycelium prevents water from penetrating the soil, and possibly because the fungus has released metabolites inimical to the plants.  The grass inside the rings sometimes grows more lush, because the fungus has liberated nitrogen by its activities. Some rings, on places such as Salisbury Plain in England, that have been under grass for many centuries, are estimated to be at least 400 years old. It is quite possible that much larger and therefore even older ones could be found. You might keep your eyes open.

Many species of agarics (perhaps as many as 60) produce fairy rings. The most obvious are those which occur in pasture, and are often generated by species of Agaricus (see Chapter 5b), or by Marasmius oreades, which is actually called the 'fairy ring mushroom' (see photograph below). Sometimes another kind of fairy ring develops around trees, as mycorrhizal fungi grow outward from the roots.

The Humungous Fungus

Some years ago, mycologists were working on a forest in Michigan where three species of Armillaria, well known pathogens, had been causing trouble.  They found that one of these species, Armillaria gallica, seemed to be monopolizing a large area of the forest - 15 hectares.  When molecular biologists checked samples from within this area, they found that all belonged to a single genet (a product of sexual reproduction).  So here was a single species of mushroom that had spread through the soil and covered an amazing 15 hectares (35 acres).  How was this achieved?

Armillaria gallica apparently had a secret weapon.  It soon became apparent that this was its rhizomorphs,  well-organized hyphal strands with conducting hyphae in the centre, and a dark protective rind on the outside, which help this fungus spread through the soil protected from the hostile influences so common in this medium.  Armillaria gallica sends out rhizomorphs through the soil in search of new food substrates.  It isn't a particularly pathogenic species, so its rhizomorphs wait patiently until a tree is weakened or dead before invading.  Having established its new base, it sends out more rhizomorphs...

Sampling established that there were about 10 tonnes of rhizomorphs in the genet.  If the fine assimilative hyphae and other structures were factored in, the total mass of the colony/genet added up to 100 tonnes - about the size of a blue whale.  It was also calculated that the colony was at least 1500 years old.

No sooner had the credentials of this fungus been established than a larger genet was discovered in Washington State.  This one belonged to Armillaria ostoyae, the common west coast species of Armillaria, and it covered an almost incredible 600 hectares (1500 acres).  Even more recently, an even larger genet of Armillaria ostoyae has been identified in the Blue Mountains of north-eastern Oregon: this one covers 2200 acres (about 900 hectares) and is estimated to be between 2,400 and 8,500 years old.   This is among the largest and most extensive organisms on earth.  Who said fungi were insignificant?

     Diploidization-Haploidization and Genetic mosaics             in Armillaria
 

It is possible that one of the reasons for the long-lasting success of these species is a rather radical departure from the genetic norm.  It has recently been discovered that in Armillaria gallica and A. tabescens, the individual nuclei in the cells of the mycelia and of the non-basidial parts of the species are haploid (not dikaryotic).  This shows that before the mushroom developed, there must have been events similar to those that usually occur only in the basidia - the fusion of two compatible nuclei and a subsequent reduction division: an extra-basidial diploidization-haploidization event.  It is obvious that for each such event, new genetic variation is introduced to the organism (crossing-over during meiosis ensures this)  So mushrooms of at least two Armillaria species are apparently mosaics of genetically distinct nuclei. When these nuclei are incorporated into basidia, and undergo yet another diploidization-haploidization event, even more diversity is introduced to the organism.  Since species of Armillaria represent some of the largest and oldest organisms on Earth, it seems possible that this 'mosaicism' may confer additional genetic flexibility on these organisms, thus contributing to their amazing success. This development of genetic mosaics has thus far been discovered in only two species of Armillaria, but it is apparent that we are at the beginning of another area of genetic exploration and discovery in the mushrooms (see Peabody et al. refs below).

An article by Tom Volk, which can also be found at: http://botit.botany.wisc.edu/toms_fungi/apr2002.html
is reproduced below.  It is written in an accessible style, and has some good pictures -- I'm pretty sure you will enjoy reading it!


The Humongous Fungus--Ten Years Later

Thomas J. Volk, Department of Biology, University of Wisconsin- La Crosse, La Crosse, WI 54601
volk.thom@uwlax.edu

Armillaria gallica fruiting bodiesHas it already been ten years? On April 2, 1992, the non-mycological world first became aware of very large fungi, thanks to the efforts of Myron Smith, Johann Bruhn, and Jim Anderson. They published a landmark article in Nature (Smith , M., J. Bruhn and J. Anderson, 1992. The fungus Armillaria bulbosa is among the largest and oldest living organisms. Nature 356:428-431), but no one expected the media blitz and the scientific interest that would follow. 

First you'll need to know a bit of background on Armillaria, also known as the Honey Mushroom. Armillaria (Fr.:Fr.) Staude is a genus of mostly pathogenic agaric fungi. Perhaps the most important aspect of the life cycle of Armillaria is the formation of rhizomorphs, conglomerations of differentiated parallel hyphae with a protective melanized black rind on the outside. The rhizomorphs are able to transport food and other materials long distances, thus allowing the fungus to grow through nutrient poor areas located between large food sources such as stumps. The rhizomorphs can also act as "scouts" for the rest of the thallus, searching for new food sources. These proliferative rhizomorphs apparently permit Armillaria colonies to spread and become quite large. Thus enters the Humongous Fungus.

The science that led to this seminal Nature publication (Smith et al. 1992) turns out to be quite interesting and very thorough. The project was actually an offshoot of a grant from the Department of Defense, which funded a project to study the possible biological effects of ELF (Extra Low Frequency) stations in the Upper Peninsula of Michigan. These ELF stations were built to communicate underground with ocean-going submarines in time of war. The humongous fungus site, which Johann Bruhn had been studying for many years, was actually one of the control sites, nowhere near the ELF stations. Historically, the site (near Crystal Falls, Michigan near the Wisconsin border) had been mostly northern red oak/white birch/ sugar maple forest, but the native trees had been harvested with more profitable red pines planted in their place. When the oaks were cut, the stumps were mostly left in the ground to rot. The oaks had been infected with Armillaria root rot, but had survived very well because they were not under any stress. However, when pines were planted, some species of Armillaria were able to kill the young pine seedlings. The particular species that garnered their attention was Armillaria bulbosa, which is now correctly known as Armillaria gallica. I'll tell you more about this taxonomic problem later.

The study of Smith et al. (1992) was performed by collecting vegetative mycelium of Armillaria by "baiting" with small pieces of poplar wood, actually popsicle stick-like tongue depressors. Since Armillaria is a wood decay fungus, the mycelium quickly colonized the tongue depressors. The labeled inoculum stick could then be easily collected. Additional subcultures, including tissues and single spore isolates, were made directly from fruiting bodies that appeared in the fall or from the black rhizomorphs and mycelial fans that are always present in the soil or on the wood, especially under the bark. The laborious process of analysis began, first with checking the mating type loci by mating on media in Petri dishes. Molecular techniques were then employed, first looking at mitochondrial DNA (mDNA) restriction patterns. These were both good markers for the study because mating type loci and mDNA restriction patterns are both highly variable within Armillaria species. Once these were determined, RAPD (Random Amplified Polymorphic DNA) and RFLP (Restriction Fragment Length Polymorphisms) markers were employed to check for additional heterozygous loci in the nuclear genome. With these several types of data, they could begin to draw maps of the area to determine the limits of each individual. One of these clones turned out to be quite large, covering 15 hectares (37 acres). Within this area, all the vegetative isolates had the same mating type , the same mDNA restriction pattern, and had the same eleven RAPD products and five RFLP-based markers, each marking a heterozygous locus. These data indicated that this 15 hectare clone is a single organism. However, some argued that this only meant that they had the same alleles at these genetic loci, bringing up the possibility that the samples were from separate, but closely related organisms (or individuals) that arose from separate matings. In their paper, Smith et al. (1992) presented some complex statistics that show that the probability of this being the case was infinitesimally small (P= 0.0013), given that all the samples share all the heterozygous markers examined. Even so, to lend even more credence to their conclusions, eventually they tested 20 RAPD and 27 nuclear restriction fragments that were found to be invariable in the large clone. By far the most likely hypothesis is that this clone reached it enormous size through vegetative growth. Thus the Armillaria clone was proven to be quite a humongous fungus. By estimating (very conservatively, I might add) the growth rate of the fungus under their natural conditions and by extrapolating the weight of the clone from smaller soil samples (again very conservatively), Smith et al. found the clone to be at least 1500 years old and weigh at least 9,700 kg (more than 21,000 pounds or 100 tons), close to the mass of an adult blue whale. They compared the mass to that of a giant redwood (Sequoiadendron giganteum) estimated to be about 1,000 tons, most of which is dead xylem tissue. The conclusion of their paper states, "This is the first report estimating the minimum size, mass, and age of an unambiguously defined fungal individual. Although the number of observations for plants and animals is much greater, members of the fungal kingdom should now be recognized as among the oldest and largest organisms on earth."

Myron Smith wrote to me recently in an email "I have often been asked something like,'what made you look for a large fungus?' Like most discoveries (and this is a point that needs to be stressed to granting agencies), we did not set out to make this discovery. Initially, (at least when Jim and I came on the scene) we wanted to find out how mitochondrial DNA was inherited in fungi in nature (Smith, Duchesne, Bruhn and Anderson, 1990). The first year we went out and sampled from a 120 x 60 m area. Nearly every sample was identical for mDNA and mating type.  The second year we extended our sampling over a 1 km transect through the area and, again, detected this one wide-spread genotype.  By extending the areas sampled in subsequent years, we were finally able to delimit the large area occupied by this genotype and then go on to show that this genotype likely represents an 'individual'." 


New York Times April 2, 2002Although they knew they were publishing a very good paper, Smith, Bruhn, and Anderson never expected what happened next. On that historic publication day, the furor began. Johann Bruhn, at that time at Michigan Technological University in Houghton, Michigan, now at the University of Missouri- Columbia, received the first of many phone calls from the media. Since it was April 2, he thought that this was a late April Fool's joke, but soon more calls began pouring in. All of the major television networks called; all of the major newspapers called from around the world. CNN called and reported that they had a plane in the air and would Johann please drive over to the site and wave so that they could take photos of the fungus. One Japanese businessman called and wanted to set up a partnership to build a boardwalk around the humongous fungus and charge people to view "the pulsating mass of fungus" that was there. Johann reports shutting himself into his office and having the secretary screen the calls one at a time as they came in. The two authors at the University of Toronto, Myron Smith (now at Carleton University in Ottawa) and Jim Anderson (still at the University of Toronto) experienced a similar media deluge. I first became aware of the media hype as I heard Jim Anderson being interviewed on US National Public Radio. You'll have to talk to the three authors to hear further interesting stories.

The media blitz lasted a month or so, then seemed to dissipate as things got back to normal. However, on May 18, 1992, it all began again. Terry Shaw, then in Colorado with the US Forest Service, and Ken Russell, of the Washington DNR, reported that they had been working on an even larger fungus, Armillaria ostoyae, that covered over 600 hectares (1500 acres, 2.5 square miles) south of Mt. Adams in southwestern Washington. The newspaper headlines read "Humongous Fungus has BIG brother out west." The fungus wars had begun. Who had the larger fungus? Questions arose as to who had better proof that theirs was a single organism. Russell and Shaw had only shown that the mating type loci were the same, but they had beautiful aerial photos showing growth of the large colony in a radial pattern, showing where it had killed the conifer trees. Smith et al. had a much more convincing argument, with several meticulous lines of genetic evidence showing without a doubt that theirs was a single clone.

Ten years later, we are still experiencing the fungus wars. In August of 2000, Catherine Parks of the US Forest Service in Oregon (along with collaborators Brennan Ferguson, Oregon State University; Tina Dreisbach, PNW Research Station, Forest Service; Greg Filip, Oregon State University; and Craig Schmitt, Wallowa-Whitman National Forest, Forest Service) reported that they had found an even larger fungus (again Armillaria ostoyae) in the Blue Mountains/ Malheur National Forest in Eastern Oregon. Their fungus is nearly 900 hectares (2,200 acres or 3.4 square miles or "as large as 1,665 football fields") and is estimated to be more than 2,400 years old. They used methods similar to those of Smith et al., including mating type analysis, but with the addition of DNA fingerprinting, not widely available in 1992. It seems likely that there are larger Armillaria clones out there somewhere. Myron Smith wrote to me: "As far as I (and I think this is true for Johann and Jim as well) was concerned, the 'Fungus wars' were a non issue; another example of sensationalistic journalism. The chance of finding "the largest fungus" is incredibly small.  Our main point was how to unambiguously identify a genetic individual.  That we did find a large individual by chance, however, suggested to us that massive, old fungi are probably not uncommon." 

One interesting offshoot of these findings of humongous fungi has been a scientific discussion of "what exactly is an organism?" Most people understand the concept of an organism in an animal, which has very carefully defined limits--and most of it is usually visible as it moves around. However, much of a typical plant and most of a typical fungus is not visible to the naked eye. In particular with fungi, the limits of the individual are not clearly defined. The large question was "are these humongous fungi acting as single organisms?" It was well proven that the genetics of various parts of the humongous fungus organism are identical, but can, for example, one part of the organism communicate with other parts of the organism? Do they share physiology? If different parts are growing through different substrates, are they supplying other parts of the fungus with missing nutrients? Several articles began to appear in the scientific literature including Gould (1992) in which he spent a great deal of time discussing populations of asexually reproducing aphids. One letter to the editor by James Bullock of Oxford University (1992) pointed out some larger clones of plants, including an aspen clone (Populus tremuloides) covering 81 hectares and over 10,000 years old. At that time Bullock did not know about the larger A. ostoyae clones.

Despite the large size of the mycelia of these humongous fungi, the fruiting bodies (mushrooms) are really quite average in size. However, during a good fruiting season, the honey mushrooms may be quite abundant, producing a widespread biomass. However, the largest single fruiting bodies are produced by perennial polypores (shelf fungi), such as Bridgeoporus nobilissimus, Rigidoporus ulmarius, and even Ganoderma applanatum. Some of these large fruiting bodies may weigh over 160 kg or 300 pounds! Certainly these are much larger than Armillaria fruiting bodies, which are typically 50-100 g each.


many fruiting bodies of Armillaria gallicaI promised to tell you something about why Armillaria gallica is the name we should use for this species rather than A. bulbosa. Armillaria species typically produce a white spore print and have attached to decurrent gills. Most species have an annulus. Delimiting species in fungi is often difficult, but in Armillaria the biological species concept, based on mating compatibility, has gained wide acceptance. Until the late 1970's Armillaria mellea (Vahl:Fr.) Kummer was considered by most researchers to be a pleiomorphic (highly variable) species with a wide host range and distribution. The pathology literature on A. mellea was extremely confusing. The fungus was considered by different researchers to be either a virulent pathogen, an opportunistic pathogen, or an innocuous saprobe. Its host range was one of the broadest known for fungi. It was clear that more than one species must be involved. Because of the difficulty with studying the basidiomata using traditional characters, other methods of study were devised. Hintikka (1973) developed a technique that allowed determination of mating (incompatibility) types in Armillaria based on culture morphology of single-spored (haploid) pairings. He and his colleagues found six biological species in Europe. The work was extended into North America, where Anderson and Ullrich (1979) demonstrated that what had been considered as Armillaria mellea in North America was actually 10 genetically isolated biological species (North American Biological Species or NABS). Anderson, Korhonen, and Ullrich (1983) found that most of the biological species of Europe (including A. gallica, NABS VII or EBS E) were also represented in North America, although the reverse was not true.

There is a bit of controversy about what to call this species. Very briefly, the name A. bulbosa Velenovský (1927) [a.k.a. A. bulbosa (Barla) Velenovský, but Barla's (1887) name A. mellea var. bulbosa was illegitimate] has a very poorly preserved type specimen. Vladimir Antonín (1986, 1990) of the Czech Republic has examined Velenovský's type specimens (preserved in a liquid fixative) and has concluded that the specimen could be any of three species. According to Marxmüller (1992) Velenovský's species is identical with A. cepistipes Velen. (1920), which has priority, being an older name (see also Termorshuizen and Arnolds, 1987). Another name proposed for this species has been Armillaria lutea Gillet, but this species lacks a type specimen, and Gillet's (1874) description could represent any one of three species. Armillaria gallica Marxmüller & Romagnesi (1987), which has an excellent type specimens and abundant cultures, is the only name that can unequivocally be assigned to European Biological Species E and NABS VII. See Volk & Burdsall (1995) for a clear explanation of this taxonomic problem.


Fungus Fest sign in Crystal Falls, Michigan No matter what we call the species, the people of Crystal Falls, Michigan have become quite enamored with the nearby humongous fungus. They now hold an annual "Fungus Fest" every September. You can buy a humongous fungus burger (unfortunately not made with Armillaria, which is in fact a delicious edible mushroom) or fungus fudge (for some reason this does not sound appealing to me...) in their restaurants. Humungus (sic) Fungus t-shirts are available in their stores, but few people from Crystal Falls have ever seen the humongous fungus or could identify its fruiting bodies. In fact, the picture on their humongous fungus web page (http://www.crystalfalls.org/humongou.htm) is clearly a Leccinum, a bolete. Yes, I have told them about it, and yes, I offered them the use of one of my pictures of Armillaria. I tried. 

Humongous Fungus U-Haul truck with Tom Volk The humongous The humongous fungus has been great publicity for the science of Mycology; we couldn't buy publicity like this. The humongous fungus even made David Letterman's Top 10 list. (see www.crystalfalls.org).

U-Haul, known for their truck rental services, got into the act in about 1997, when they contacted me for more information about the humongous fungus. Famous for publicizing some of the more bizarre "roadside attractions" on their trucks, U-Haul planned on putting the humongous fungus on some of their trucks to honor the state of Michigan. Through my web page, they contacted me and asked to use one of my pictures. I consented, hoping to help promote mycology to the masses. A month later they sent me a sample drawing for my approval-and the mushrooms were PINK! I diplomatically pointed out that in fact the mushrooms were not pink and that they should put them on the truck in their natural tan/brown/yellow color, since there were thousands of professional and amateur mycologists throughout North America who would know that their fungi were discolored. The U-Haul people replied back that they had taken some "artistic license" with the color, since they thought the natural color was not exciting enough. Sheesh. So now there are several hundred U-Haul trucks around the continent with pink Armillaria fruiting bodies on them. U-Haul now even has a website about the humongous fungus (Anonymous 2002A). It's exciting for me to see one of my pictures (well, sort of one of my pictures...) on one of the 500 or so Humongous Fungus trucks as I drive down the highway-- I've seen the humongous fungus trucks from Maine to California, from Minneapolis to Houston. I like to think it's helping to make the public more aware of fungi and mycology. Myron Smith again wrote: "I like to think that what grabbed the imagination of the public in this case was the idea that there are common, unseen things all around us that are magnificent.  Of course, the mental image of a large, old fungus lumbering over the countryside is also bizarre and wonderful."

The humongous fungus continues to be a great boon for educating non-mycologists on the importance of fungi in their lives. The important publicity generated by the work of Myron Smith, Johann Bruhn, Jim Anderson, and the others that followed continues to speak well for the science of Mycology. Even more humongous fungi will no doubt continue to be found. As mycologists we have a multitude of Armillaria researchers to thank for putting mycology in the news in favorable light for a very long period of time. Mycology is not likely to get such great publicity again in our lifetimes. But you never know...

Acknowledgments: Thanks to Myron Smith, Jim Anderson, Dan Czederpiltz, and Sean Westmoreland for reading the manuscript and making helpful suggestions. 


References
  • Anderson JB, Ullrich RC (1979) Biological species of Armillaria in North America. Mycologia 71: 402?414
  • Anderson JB, Korhonen K, Ullrich RC (1980) Relationships between European and North American biological species of Armillaria mellea. Exper. Mycol. 4: 87?95.
  • Anonymous. 2002A.U-Haul Humongous fungus web page http://www.uhaul.com/supergraphics/fungus/
  • Anonymous. 2002B. Crystal Falls Fungus Fest webpages. http://www.crystalfalls.org/FUNGUS%20FEST.htmhttp://www.crystalfalls.org/humongou.htm
  • Antonín V (1986) Studies in annulate species of Armillaria - I Study of type-specimens of Armillaria cepaestipes Velenovský. Ceská Mykol. (Praha) 40: 38-40.
  • Antonín V (1990) Studies in annulate species of Armillaria - III. Species described by Josef Velenovský. Acta Mus. Moraviae Sci. Nat. 75: 129-132.
  • Barla G (1887) Bull. Soc. Mycol. France 3:143
  • Dodge SR (2001) An Even More Humongous Fungus, http://www.fs.fed.us/pnw/news/fungus.htm
  • Bollock J (1992) Huge organisms. New Scientist 30 May, p. 54
  • Gillet C-C (1874) Les Hymenomycčtes ou description de tous les champignons (Fungi) qui croissent en France.
  • Gould SJ (1992) A humongous Fungus Among Us. Natural History, July p. 10-14.
  • Herink J (1973) Taxonomie Václavky Obecné? Armillaria mellea (Vahl. ex Fr.) Kumm. pp. 21?48. In: Vysoká Skola Zem_d_lska V Brné. Vyznamenaná Rádem Prace BRNO; J. Hasek, ed. Brno: Lesnicka fakulta VSZ.
  • Hintikka V (1973) A note on the polarity of Armillariella mellea. Karstenia 13:32?39.
  • Marxmüller H (1992) Some notes on the Taxonomy and Nomenclature of five European Armillaria species. Mycotaxon 44 (2): 267-274.
  • Marxmüller H, Romagnesi H (1987) Bull. Soc. Mycol. France 103:152
  • Shaw CT, Kile GA (1991) Armillaria root disease. Agriculture handbook 691, 233 pp.
  • Smith M, Bruhn J, Anderson J (1992) The fungus Armillaria bulbosa is among the largest and oldest living organisms. Nature 356:428-431
  • Smith ML, Duchesne LC, Bruhn JN, Anderson JB (1990)  Mitochondrial genetics in a natural population of the plant pathogen Armillaria.  Genetics 126: 575-582
  • Termorshuizen A, Arnolds E (1987) On the nomenclature of the European species of the Armillaria mellea group. Mycotaxon 30: 101-116.
  • Velenovský J (1920) Ceské Houby 1: 1-950
  • Velenovský J (1927) Václavka hlíznatá (Armillaria bulbosa Barla) Mykologia (Praha) 4: 116-117; translation to English by Vladimír Antonín. (Pers. Comm.)
  • Volk TJ, Burdsall HH Jr. (1995) Nomenclatural study of Armillaria and Armillariella species (Basidiomycotina, Tricholomataceae) Fungiflora, Oslo, Norway: Synopsis Fungorum 8, 121 pp. ISBN 82-90724-14-4
  • Volk TJ (1995-2002) Tom Volk's fungi. TomVolkFungi.net

What's an ATBI ?

Of course, you can't do fungal ecology unless you know what fungi are present.  There is almost certainly no habitat in the world whose fungi have been fully enumerated.  A group of 22 mycologists gathered in Costa Rica in 1995 and came up with a strategy for isolating and identifying all of the fungi (an estimated 50,000) in a particular habitat (the Guanacaste Conservation Area) -- an All-Taxa Biodiversity Inventory for fungi (Rossman et al. [eds.] 1998 -- see reference below)This ambitious plan called for a staff of 100,  $1 million worth of agar media, 1.8 million slants to isolate the endophytic fungi alone... Unfortunately all this would have cost about  US$25 million, so it hasn't been done.  But the need remains, and the general lack of knowledge about fungi means that they are not usually considered when conservation issues are raised.  Perhaps you can help to change that.  

A less ambitious ATBI is now under way in Great Smoky Mountains National Park, but it is a long-term endeavour -- visit the web site at http://www.discoverlife.org/
As of September 2003, about 2,200 fungi had been recorded for the Smokies ATBI.  This is hundreds more than were known from this area prior to the study, and the number is expected to rise inexorably as the study continues... 

A well-known and scientifically ambitious couple, Oluna and Adolf Ceska, living in Victoria, BC, began in 2004 to compile an inventory of the fungi of Observatory Hill, a largely forested area, 71.4 ha in area and 224m high, just north of Victoria. After several years, and by dint of making almost 200 trips to the hill, they have as of late 2012 collected more than 1,100 species, and the study continues... They have found a number of extremely rare species, and some undescribed taxa.  Theirs is now probably the most intensive and species-rich study for such a small area in the history of mycology. An account of the study can be accessed at:
http://www.geog.ubc.ca/biodiversity/eflora/macrofungi_observatory_hill.html
and
http://www.goert.ca/documents/Macrofungi-Observatory-Hill-2011-2012.pdf

During the same years the Ceskas also participated in a multi-year study of Clayoquot Sound, including Pacific Rim National Park, other team members being Christine Roberts, Paul Kroeger and Bryce Kendrick (Roberts et al. 2004).  They found 551 species, only 28 being collected in all years, and 308 species being found in only one year.

A similar study in the Haida Gwaii archipelago, with the same 5 mycologists, visiting the islands 2 or 3 times per year, found over 600 taxa, and led to the publication of a book 'The Outer Spores - Mushrooms of Haida Gwaii' (Kroeger et al. 2012). 

Now we can approach this problem in a completely new way, as this article in PNAS describes:

... researchers traveled to 26 pine forests across North America and collected 10-cm-deep soil cores, more than 600 in all. Within hours of collection, and with the assistance of local scientists and universities, they preserved the samples to extract and isolate the fungal DNA. The researchers then used modern genomic tools to sequence unique stretches of the environmental DNA that can be used as barcodes to identify all of the fungal species present in each sample.

The sequencing revealed more than 10,000 species of fungi, which the researchers then analyzed to determine biodiversity, distribution, and function by geographical location and soil depth. Interestingly, author Peay said, there was very little overlap in the fungal species from region to region; East Coast fungi didn't show up on the West Coast or Midwest, and vice versa.

"People often assume that similar habitats in, say, North Carolina and California would have similar fungi, but this is the opposite of what we find," an author said. "What’s more interesting, despite the fact that soil fungal communities in Florida and Alaska might have no fungi in common, you find that many of the processes and the functional rates are convergent. The same jobs exist, just different species are doing them."

The team found this to be particularly true when comparing the functionality of fungi at different strata of the core samples. Even though the samples were collected thousands of miles apart, fungi near the top all performed the same task; similarly, bottom fungi performed very similar functions across the continent.

We need many more similar studies to establish baseline data for North American fungi, such as have existed for many years in Europe, which may give us warnings about decline and loss of species resulting from anthropogenic influences (habitat loss, climate change).

And having outlined that encouraging state of affairs, we must turn the page to another, completely different aspect of mycology which came to prominence in the middle of the 19th century, and has remained front and centre ever since...  

         Go to Chapter 12
            Go to Table of Contents

© Mycologue publications 2015


Further Reading on Fungal Ecology

Baerlocher F (1992) (ed) The Ecology of Aquatic Hyphomycetes. Ecological Studies 94.  Springer Verlag.

Baerlocher F, Kendrick B (1974) Dynamics of the fungal population on leaves in a stream. J. Ecology 62: 761-791.

Baerlocher F, Kendrick B (1981) The role of aquatic hyphomycetes in the trophic structure of streams. pp. 743-760 (in) The Fungal Community: its Organization and Role in the Ecosystem. (eds.) E.T. Wicklow ET, Carroll GC. Marcel Dekker, New York.

Bell A (1983) Dung Fungi: an illustrated guide to coprophilous fungi in New Zealand. Victoria University Press, Wellington.

Cannon PF (1995) An ATBI - How to find one and what to do with it.  Inoculum 46: 1-4

Deighton J (2003) Fungi in Ecosystem Processes. Mycology Series No. 17.    424pp  Marcel Dekker.

Doveri F (2004) Fungi Fimicoli Italici: A guide to the recognition of basidiomycetes and ascomycetes living on faecal material.  1104 pp.  Assoc. Micol. Besadola

Frankland JC (1998)  Fungal succession - unravelling the unpredictable.   Mycol. Res. 102: 1-15

Hudson HJ (1980) Fungal Saprophytism. 2nd Edn. Arnold, London.

Ingold CT (1966) The tetraradiate aquatic fungal spore. Mycologia 58: 43-56.

Ingold CT (1975) Guide to Aquatic Hyphomycetes. Freshwater Biological Assoc. Publ. # 30. 96 pp.  Ambleside.

Kendrick B (1958) Microfungi in pine litter.  Nature 181: 432.

Kendrick B (1958) Helicoma monospora sp. nov. from pine litter.
Trans. Brit. mycol. Soc. 41: 446-448. [later made the type species of  Slimacomyces Minter]

Kendrick B (1958) Sympodiella, a new hyphomycete genus.
Trans. Brit. mycol. Soc. 41: 519-521.

Kendrick B (1959) The time factor in the decomposition of coniferous leaf litter. Can. J. Bot. 37: 907-912.

Kendrick B (1961) Hyphomycetes of conifer leaf litter. Thysanophora gen. nov. Can. J. Bot. 39: 817-832.

Kendrick B, Burges A (1962) Biological aspects of the decay of Pinus sylvestris leaf litter.    Nova Hedwigia 4: 313-342.

Kroeger P, Kendrick B, Ceska O, Roberts C (2012) The Outer Spores: Mushrooms of Haida Gwaii.  Mycologue Publications, Sidney, BC. and Haida Gwaii Museum, Skidegate.   http://www.mycolog.com/mycologueproducts.html

Michaelides J, Kendrick B (1982) The bubble-trap propagules of Beverwykella, Helicoon and other aero-aquatic fungi. Mycotaxon 14: 247-260.

Nikolcheva LG, Bourque T, Baerlocher F (2005) Fungal diversity during initial stages of leaf decomposition in a stream. Mycol. Res. 109: 246-253.

Paulus B, Gadek P, Hyde KD (2003) Estimation of fungal biodiversity in tropical rainforest leaf litter using particle filtration: the effects of leaf storage and surface treatment.  Mycol. Res. 107: 748-756.

Peabody RB, Peabody DC, Sicard KM (2000) A genetic mosaic in the fruiting stage of Armillaria gallica. Fungal Genetics and Biology 29: 72-80.

Peabody RB, Peabody DC, Tyrrell MG, Edenburn-MacQueen E, Howdy RP, Semelrath KM (2005) Haploid vegetative mycelia of Armillaria gallica show among-cell-line variation for growth and phenotypic plasticity. Mycologia 97: 777-787.

Price PW (1988) An overview of organismal interactions in ecosystems in evolutionary and ecological time. Agriculture, Ecosystems and Environment 24: 369-377.

Richardson MJ, Watling R (1982) Keys to fungi on dung (Revised Edition). British Mycological Society, Cambridge.

Richardson MJ (2001) Diversity and occurrence of coprophilous fungi.  Mycol. Res. 105: 387-402. 

Roberts C, Ceska O, Kroeger P, Kendrick B (2004) Macrofungi from six habitats over five years in Clayoquot Sound, Vancouver Island. Can. J. Bot. 82: 1518-1538.

Rossman AY, Tulloss RE, O'Dell TE, Thorn RG, (eds) (1998). Protocols for an All Taxa Biodiversity Inventory in a Costa Rican Conservation Area. Parkway Publishers, Boone, North Carolina, U.S.A.

Seifert, K, Kendrick B, Murase G (1983) A Key to Hyphomycetes on Dung. University of Waterloo Biology Series, 27. Department of Biology, University of Waterloo, Waterloo.

Straatsma G, Ayer F, Egli S (2001) Species richness, abundance and phenology of fungal fruit bodies over 21 years in a Swiss forest plot.  Mycol. Res. 101: 515-523.

Talbot JM, Bruns TD, Taylor JW, Smith DP, Branco S, Glassman SI , Erlandson S, Vilgalys R, Liao H-L, Smith ME, Peay KG (2014) Endemism and functional convergence across the North American soil mycobiome  Proc Natl Acad Sci USA 111: 6341-6346.

 Tofts RJ, Orton PD (1998) The species accumulation curve for agarics and boleti from a Caledonian pinewood.  Mycologist 12: 98-102.

Webster J (1970) Coprophilous fungi. Transactions of the British Mycological Society 54: 161-180.

Webster J, Descals E (1981) Morphology, distribution, and ecology of conidial fungi in freshwater habitats. pp. 295-355 (in) Biology of Conidial Fungi. Vol. 1 (eds.) Cole GT, Kendrick B. Academic Press, New York.